Dom Finds Maria - Gears Of War 2
Gears Of War 2 isn't the sort of game you expect to find a tear-jerking moment in, but the scene where Dom finds his lost wife Maria definitely qualifies. Dom had heard that she'd been captured by the Locust, and he was desperate to locate her. When he finally finds her, it takes Marcus pointing out what's happened for him (and us) to see it. Even worse, Dom realizes that she's a broken husk of what she once was, and he can't save her. Finally, we hear the shot that Dom uses to put her out of her misery from offscreen. It's a moment that you can't forget, and it colors Dom's character for the rest of the series.